Journal: Acta Crystallographica. Section F, Structural Biology Communications

Article Title: The structure of a glycoside hydrolase 29 family member from a rumen bacterium reveals unique, dual carbohydrate-binding domains

doi: 10.1107/S2053230X16014072

Figure Lengend Snippet: Macromolecule-production information

Article Snippet: The protein was concentrated (at room temperature) using a 10 kDa molecular-weight cutoff ultrafiltration centrifugation column (Sartorius, Germany), further purified by gel filtration on a S200 16/60 size-exclusion column (GE Healthcare, Germany) in 50 m M Tris pH 7.5, 100 m M NaCl buffer and concentrated. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Source organism Unknown DNA source Unknown Forward primer † Gene-specific att B1 primer as designed by GeneArt for Gateway cloning Reverse primer Gene-specific att B2 primer as designed by GeneArt for Gateway cloning Cloning vector pENTR221 Expression vector pDEST17 Expression host E. coli BL21 (DE3) Complete amino-acid sequence of the construct produced MSYYHHHHHHLESTSLYKKAGF ENLYFQS AQVEPCGPVPTENQLRWQDMEMYAFIHYSLNTYTDEEWGYGNEDPQLFNPSSLDCRQWARVCKQAGMRGIIFTAKHHCGFCMWPSAYTEYSVKNSPWKNGKGDVVRELADACREEGLKFAVYLSPWDRNHPAYGQPAYVAYFRNQLRELLTNYGEIFEVWFDGANGGDGWYGGANETRKIDRTTYYQWPETYKMIRQLQPNCLIWNDGSDRGDLRWVGTEAGNVGETNWSLLNHDGEVEWHMLHYGLENGDSWVPGETNTSIRPGWFYHDTENEHVKSLSKLMDTYYKSVGRNSTLLLNFPIAPNGRIHPNDSLRGIAFKKMIGEVFRKNLAEKARTQTKGDETVIDFGKPTTFNRFLAEEDIRYGQRVKKFLLEAEINGQWQQLKDALVENGDGLTTIGHRRIICFPTVNATKLRFTVVNTKCEPFIKKLGVYLAPELTADIPDAGEKKSSNLHLFFSSPTQMMIDWETEQTITSFRYLPPQESKDGTVTHYTLWASTDWSNWTKLASGEFSNVVNNPIWQTIKFQPVRAKILKLDADRLATGNRMAYGDVEVNLKLNIEN Open in a separate window † The att B1 forward primer contained an upstream rTEV cleavage site (underlined) caption a8 Macromolecule-production information

Techniques: Clone Assay, Plasmid Preparation, Expressing, Sequencing, Construct, Produced

Journal: Brazilian Journal of Microbiology

Article Title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification and antifungal susceptibility analysis of Candida species isolated from patients with invasive yeast infections in five university hospitals

doi: 10.1007/s42770-018-0027-0

Figure Lengend Snippet: Comparison of results regarding yeast identifications via Bruker Biotyper and Vitek 2 Compact

Article Snippet: As shown in Table , five isolates were incorrectly identified by VITEK 2 YST, and one C . pelliculosa isolate was misidentified as C . parapsilosis on Bruker MS. One C . metapsilosis , one C . guilliermondii , one C . lusitaniae , and one C . krusei isolate were identified correctly; however, these results were unreliable, based on the low scores of < 1.7. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Species Bruker Biotyper (score) Vitek 2 Compact (ID lever %) C . metapsilosis C . metapsilosis (1.572) C . parapsilosis (91%) Meyerozyma guilliermondii C . guilliermondii (1.602) C . famata (59%)/ C . guilliermondii (41%) C . lusitaniae C . lusitaniae (1.515) C . famata (50%)/ C . lusitaniae (50%) C . krusei C . krusei (1.637) C . lusitaniae (50%)/ C . krusei (50%) C . pelliculosa C . pelliculosa (1.431) C . parapsilosis (50%)/ C . pelliculosa (50%) Open in a separate window Details of the discordant results between Bruker Biotyper and Vitek 2 Compact The origin and distribution of pathogenic yeasts Of 216 patients with invasive fungal infections, 130 were male and 86 were female (male:female = 1.5:1), and the age range was 0.004 to 88 years (50.5 ± 22.6 years).

Techniques:

Journal: Brazilian Journal of Microbiology

Article Title: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric identification and antifungal susceptibility analysis of Candida species isolated from patients with invasive yeast infections in five university hospitals

doi: 10.1007/s42770-018-0027-0

Figure Lengend Snippet: Details of the discordant results between Bruker Biotyper and Vitek 2 Compact

Article Snippet: As shown in Table , five isolates were incorrectly identified by VITEK 2 YST, and one C . pelliculosa isolate was misidentified as C . parapsilosis on Bruker MS. One C . metapsilosis , one C . guilliermondii , one C . lusitaniae , and one C . krusei isolate were identified correctly; however, these results were unreliable, based on the low scores of < 1.7. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Species Bruker Biotyper (score) Vitek 2 Compact (ID lever %) C . metapsilosis C . metapsilosis (1.572) C . parapsilosis (91%) Meyerozyma guilliermondii C . guilliermondii (1.602) C . famata (59%)/ C . guilliermondii (41%) C . lusitaniae C . lusitaniae (1.515) C . famata (50%)/ C . lusitaniae (50%) C . krusei C . krusei (1.637) C . lusitaniae (50%)/ C . krusei (50%) C . pelliculosa C . pelliculosa (1.431) C . parapsilosis (50%)/ C . pelliculosa (50%) Open in a separate window Details of the discordant results between Bruker Biotyper and Vitek 2 Compact The origin and distribution of pathogenic yeasts Of 216 patients with invasive fungal infections, 130 were male and 86 were female (male:female = 1.5:1), and the age range was 0.004 to 88 years (50.5 ± 22.6 years).

Techniques:

Journal: The Clinical Biochemist Reviews

Article Title: An Evidence-Based Approach to the Assessment of Heart-Type Fatty Acid Binding Protein in Acute Coronary Syndrome

doi:

Figure Lengend Snippet: Prognostic value of H-FABP in studies recruiting exclusively patients with ‘confirmed’ acute coronary syndrome.

Article Snippet: Circulation 2006;114:550–7. table ft1 table-wrap mode="anchored" t5 caption a7 Author and year of publication N H-FABP assay Clinical setting MI (%) Follow-up duration Biomarkers measured Key findings Ishii et al. 2005 44 328 Dainippon Consecutive CCU admissions, sample on admission 73.5% 6 mths TnT Increased H-FABP was independently associated with cardiac events (cardiac death or non-fatal MI) RR=9; p=0.0004 Erlikh et al. 2005 45 203 Hycult Sample 6 h from symptom onset NA 12 mths TnI, CKMB Elevated H-FABP was independent predictor of death or non-fatal MI, OR 2.45 95% CI (1.1–5.2); p=0.02 O’Donoghue et al. 2006 32 2287 In-house (Alere San Diego) Clinical trial subset, mean time to randomisation = 41h 55% 10 mths TnI, BNP, myoglobin Elevated H-FABP was an independent predictor of death, recurrent MI, congestive heart failure or the composite of these end points (HR, 1.9; 95% CI, 1.3 to 2.7).

Techniques:

Journal:

Article Title: Comparative In Vitro Activity of ABT-773, a Novel Antibacterial Ketolide

doi: 10.1128/AAC.45.7.2163-2168.2001

Figure Lengend Snippet: Minimum bactericidal activities of ABT-773 a

Article Snippet: In contrast, ABT-773 was bacteriostatic for a strain of S. aureus . table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Species ABT-773 Erythromycin Ciprofloxacin MIC MBC MIC MBC MIC MBC S. pneumoniae ATCC 6303 No serum 0.002 0.004 0.03 0.12 1 1 Serum 0.004 0.008 0.06 2 1 2 H. influenzae 1882 No serum 0.5 1 4 8 0.004 0.008 Serum 0.5 1 1 2 0.004 0.004 M. catarrhalis 2604 No serum 0.25 0.5 0.25 0.5 0.12 0.12 Serum 0.12 0.12 0.12 0.12 0.12 0.12 S. aureus ATCC 6538P No serum 0.03 >1 0.25 >8 0.12 0.25 Serum 0.015 >1 0.03 >1 0.12 0.5 Open in a separate window a In micrograms per milliliter.

Techniques: